Application of Sphenoid Sinus Morphological Characteristics in Sex Estimation Based on Magnetic Resonance Imaging Images / Aplicación de las Características Morfológicas del Seno Esfenoidal en la Estimación del Sexo Basada en Imágenes de Resonancia Magnética

SUMMARY: Sex determination of unknown persons plays an important role in forensic science. As most bones used for sex determination are recovered in incomplete state, it is often necessary to use bones that are recovered intact e.g., the sphenoid sinus. This study aimed to evaluate the diagnostic value of sphenoid sinuses dimensions for sex determination using Magnetic Resonance Imaging (MRI) images in Chinese adults. MRI images of 79 sphenoid sinuses (from 44 men and 35 women) were retrospectively selected. The height, anterior-posterior diameter, area, and perimeter were measured in the midsagittal view of the sphenoid sinuses. All data were subjected to descriptive and discriminative functional analysis with unpaired t-test and canonical discriminant. Comparison between male and female groups showed significant statistical differences regarding the height, anterior-posterior diameter, area, and perimeter of sphenoid sinuses. The predictive accuracy rate of the sphenoid sinus to identify sex was 63.6 % in males and 62.9 % in females with an overall accuracy of 63.3 %. This study proposed the importance of sexual dimorphism of sphenoid sinus dimensions, especially if other methods are not available. It suggested using MRI in forensics science thus obviating the complete dependence on the usage of conventional computed tomography (CT) and facilitating the study of forensic anatomy at the level of soft tissue.

La determinación del sexo de personas desconocidas juega un papel importante en la ciencia forense. Como la mayoría de los huesos utilizados para la determinación del sexo se recuperan en un estado incompleto, a menudo es necesario utilizar huesos recuperados intactos, por ejemplo, el seno esfenoidal. Este estudio tuvo como objetivo evaluar el valor diagnóstico de las dimensiones de los senos esfenoidales para la determinación del sexo utilizando imágenes de resonancia magnética en individuos adultos chinos. Se seleccionaron retrospectivamente imágenes de resonancia magnética de 79 senos esfenoidales (de 44 hombres y 35 mujeres). La altura, el diámetro anteroposterior, el área y el perímetro de los senos esfenoidales, se midieron en vista mediana sagital. Todos los datos se sometieron a análisis funcional descriptivo y discriminativo con prueba t no pareada y discriminante canónico. La comparación entre los grupos de hombres y mujeres mostró diferencias estadísticas significativas en cuanto a la altura, el diámetro anteroposterior, el área y el perímetro de los senos esfenoidales. La tasa de precisión predictiva del seno esfenoidal para identificar el sexo fue del 63,6 % en hombres y del 62,9 % en mujeres, con una precisión general del 63,3 %. Este estudio propuso la importancia del dimorfismo sexual de las dimensiones del seno esfenoidal, especialmente si no se dispone de otros métodos. Se sugiere utilizar la resonancia magnética en la ciencia forense, obviando así la dependencia total del uso de la tomografía computarizada convencional y facilitando con esto el estudio de la anatomía forense a nivel de los tejidos blandos.

Analysis of the autoimmune response to BP180 in Chinese stroke patients

Abstract Background Significant association between bullous pemphigoid (BP) and stroke has been reported. This study aimed to evaluate the level of anti-BP180 antibody in stroke patients to explore the relationship between BP and stroke in their pathogenesis. Methods We collected serum samples from stroke patients and matched controls between February 2019 and June 2020. The anti-BP180 antibody levels were measured by enzyme-linked immunosorbent assay (ELISA). Results A total of 1183 stroke patients including 970 with cerebral infarction (CI), 192 with intracerebral hemorrhage (ICH), 21 with CI and ICH, and 855 controls were enrolled in this study. Anti-BP180 autoantibody values were significantly higher in stroke patients than in controls (p < 0.001). Anti-BP180 autoantibody-positive rates were 12.51% (148) in stroke patients and 4.68% (40) in controls (p < 0.001, OR = 2.65). In anti-BP180 autoantibody-positive subjects, the values were significantly higher in stroke patients than in controls (p < 0.001). However, only 10 (6.76%) stroke patients and 3 (7.5%) controls had high values (> 100 RU/mL) (p = 0.87). Stratified analysis showed that anti-BP180 antibody positive rates were independent of age, sex, and stroke subtypes in the stroke group. Positive rates in patients with both CI and ICH were nearly two times higher than those in patients with either CI or ICH alone (p = 0.11, OR = 1.94). Study limitations This study had a limited sample size and lacked quantitative criteria for stroke severity. Conclusions Anti-BP180 antibody values and positive rates were higher in stroke patients than in controls, suggesting that stroke patients may have higher of developing BP.

Clinical efficacy and safety of tumor-specific individualized multi-target DC-CIK in the treatment of advanced non-small cell lung cancer / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：评价肿瘤特异性个体化多靶点树突状细胞-细胞因子诱导的杀伤细胞（DC-CIK）治疗晚期非小细胞肺癌（NSCLC）患者的临床疗效和安全性。方法：回顾性分析2019年10月1日至2022年10月31日东部战区总医院生物治疗科行肿瘤特异性个体化多靶点DC-CIK治疗晚期NSCLC患者的临床资料。统计NSCLC患者的临床疗效和不良反应，分析治疗前后血清中肿瘤标志物的变化，FCM检测患者治疗前后的淋巴细胞亚群和各种细胞因子的表达情况，用质谱仪检测治疗前后靶点的变化。结果: 共入组52例晚期NSCLC患者，其中女性21例、男性31例；年龄32~71岁，平均年龄（50.97±10.72）岁，中位年龄47.5岁。经DC-CIK治疗后，CR 0例，PR 0例，SD 27例，PD 25例。与治疗前比较，DC-CIK治疗后：（1）CEA和CYFRA21-1水平无显著改变，CA125水平显著低于治疗前（P<0.01）；（2）治疗后患者淋巴细胞亚群无显著变化；（3）治疗后患者外周血IL-2、IL-4、IFN-γ和TNF-α水平显著升高（均P<0.01），IL-6、IL-10及IL-17水平无明显变化；（4）治疗后靶点数下降明显。DC-CIK治疗过程中无严重不良反应发生。结论: 晚期NSCLC患者行肿瘤特异性个体化多靶点自体DC-CIK治疗是安全的，能使患者产生抗肿瘤免疫反应并得到一定的临床获益。

A cohort study of infant development and health in Tianjin / 中国学校卫生

Objective@#To investigate the current situation of obesity and related metabolic abnormalities among preschool children, so as to provide theoretical support for future intervention.@*Methods@#A cohort of 3 952 children, born in Tianjin and enrolled in the kindergarten from September 2017 to October 2018, were selected to conduct a baseline survey and a three-year follow-up (questionnaire survey, physical examination and laboratory testing). At the same time, a two-way cohort study was conducted to retrospectively collect maternal prenatal examination, delivery and regular physical examination information of children from birth to preschool age from Tianjin Maternal and Child Health Information System.@*Results@#A total of 3 935, 3 654 and 2 739 children completed the follow up in the primary, middle and senior classes of kindergarten respectively. The height and weight of pre-school children increased with age, while the percentage of body fat decreased with age ( β-trend =-0.74, P <0.01). During three-year follow up, height, weight and body mass index of boys were higher than girls (P<0.05), while the percentage of body fat was lower than girls (primary class: 17.5%,18.5%; middle class: 16.4%,17.2%; senior class: 16.1%,17.1%, P <0.05). The detection rate of overweight (including obesity) and obesity increased with age( χ 2 trend were 15.51,38.72, P <0.05). The total detection rate of obesity increased from 5.4% at the baseline level to 9.6%. Laboratory test results showed that the detection rates of fasting blood glucose of boys were higher than that of girls in primary class, but blood lipid abnormalities were in the opposite (glucose: 7.7%, 4.8 %; lipid: 23.8% , 27.7%)( χ 2=12.01, 6.63, P <0.05).@*Conclusion@#The study has established a large growth cohort starting from the early embryonic stage, which will help to establish the strategies to promote children s health and prevent obesity and chronic diseases from multidimensional perspectives.

Improving physical function by focusing on sports archery training / Aprimoramento da função física pelo foco no treinamento com arco esportivo / Mejora de la función física centrándose en el entrenamiento del arco deportivo

ABSTRACT Introduction: Physical training of high-level archers is a very critical point. It is now known that the ultimate goal of athletes' training is to achieve a good competitive state in their physical training. This has revealed that the relationship between athletes' attention stability and their performance in archery competitions also needs to be explored. Objective: Analyze the fitness training methods for Chinese archers. In addition, this paper also explores the relationship between attention and performance in competitions. Methods: By random sampling method, 24 archery athletes were investigated. They were divided into three groups: A, B, and C. Group A represented excellent scores (8), group B with good scores (8), and group C with average scores (8). Statistical methods were used to analyze and infer the data. Using the SPSS program, mathematical statistics were performed to objectify the data collected. Results: Athletes in group A had the greatest increase in attention after training, showing statistical significance (P<0.01). The comparison between groups B and C also showed statistical significance (P<0.01). Significant differences were found in the stability of attention between the experimental and control groups. Conclusion: The sport bow practice method can effectively improve athletes' concentration. This training method showed a significant effect on improving archers' attention and concentration. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O treinamento físico dos arqueiros de alto nível é um ponto muito crítico e atualmente sabe-se que o objetivo final do treinamento dos atletas é alcançar um bom estado competitivo em seu treinamento físico. Isso tem revelado que a relação entre a estabilidade da atenção dos atletas e seu desempenho nas competições com arco também precisa ser explorada. Objetivo: Analisar os métodos de treinamento de aptidão física para os arqueiros chineses. Além disso, este documento também explora a relação entre a atenção e o desempenho nas competições. Métodos: Pelo método de amostragem aleatória investigou-se 24 atletas de arco e flecha. Eles foram divididos em três grupos: A, B, e C. O grupo A representando excelentes resultados (8); O grupo B com boas notas (8), e o grupo C com notas médias (8). Utilizou-se métodos estatísticos para analisar e inferir os dados. Utilizando o programa SPSS, foram realizadas as estatísticas matemáticas para objetivar os dados coletados. Resultados: Os atletas do grupo A tiveram o maior aumento na atenção após o treinamento, apresentando significância estatística (P<0,01). A comparação entre os grupos B e C também apresentou significância estatística significativa (P<0,01). Foram encontradas diferenças significativas na estabilidade da atenção entre os grupos experimental e controle. Conclusão: O método de prática com arco esportivo pode efetivamente melhorar a concentração dos atletas. Este método de treinamento apresentou um efeito significativo na melhoria da atenção e concentração dos arqueiros. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: La preparación física de los arqueros de alto nivel es un punto muy crítico y hoy en día se sabe que el objetivo final del entrenamiento de los deportistas es alcanzar un buen estado competitivo en su preparación física. Esto ha revelado que la relación entre la estabilidad de la atención de los atletas y su rendimiento en las competiciones de tiro con arco también debe ser explorada. Objetivo: Analizar los métodos de entrenamiento físico de los arqueros chinos. Además, este trabajo también explora la relación entre la atención y el rendimiento en las competiciones. Métodos: Mediante un método de muestreo aleatorio se investigaron 24 atletas de tiro con arco. Se dividieron en tres grupos: A, B y C. El grupo A, que representa resultados excelentes (8); el grupo B, con buenos resultados (8), y el grupo C, con resultados medios (8). Se utilizaron métodos estadísticos para analizar e inferir los datos. Utilizando el programa SPSS, se realizaron estadísticas matemáticas para objetivar los datos recogidos. Resultados: Los atletas del grupo A tuvieron el mayor aumento de la atención después del entrenamiento, mostrando significación estadística (P<0,01). La comparación entre los grupos B y C también presentó significación estadística (P<0,01). Se encontraron diferencias significativas en la estabilidad de la atención entre los grupos experimental y de control. Conclusión: El método de práctica del arco deportivo puede mejorar eficazmente la concentración de los deportistas. Este método de entrenamiento mostró un efecto significativo en la mejora de la atención y la concentración de los arqueros. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Reaction speed training in volleyball / Treinamento de velocidade de reação no voleibol / Entrenamiento de velocidad de reacción en el voleibol

ABSTRACT Introduction: Volleyball is a team sport where winning the confrontation at the net largely determines characteristics. Volleyball players must constantly strive to improve team cooperation, defensive ability, and speed skills to improve their ability to perform over the net. Objective: Establish a fixed intensity load model for speed training on the athlete's visual response in volleyball. Methods: This paper selected several volleyball players as research subjects, randomly divided into two groups (regular training and experimental groups). The experimental group was subjected to a visual response speed training protocol based on routine training. Mathematical statistics were employed to analyze the athletes' reaction speed and defensive ability against the net. Results: The qualities analyzed, mainly movement speed, swing speed, agility, and endurance for fast swing and fast movement, showed a significant differential after the intervention. The speed response was concentrated in the center of body balance. The lower limbs increased the endurance for speed. After visual reaction speed training, the experimental group's ability to cope with attacks on the net was statistically superior (P<0.05). Conclusion: The proposed protocol can improve the reaction speed training of volleyball players. Volleyball players should reinforce reaction speed training daily to perform better close to the net. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O voleibol é um esporte coletivo e suas características de vitória são determinadas em grande parte pelo confronto em rede. Os jogadores de vôlei devem se esforçar constantemente para melhorar a cooperação da equipe, a capacidade defensiva e habilidades de velocidade para melhorar a capacidade de atuarem pela a rede. Objetivo: Estabelecer um modelo de carga de intensidade fixa para o treinamento de velocidade na resposta visual do atleta em voleibol. Métodos: Este trabalho seleciona vários jogadores de vôlei como objetos de pesquisa, divididos aleatoriamente em dois grupos (grupos de treinamento regular e grupos experimentais). O grupo experimental teve o diferencial de ser submetido a um protocolo treinamento de velocidade de resposta visual baseado no treinamento de rotina. Estatísticas matemáticas foram empregadas para analisar a velocidade de reação dos atletas e a capacidade defensiva contra a rede. Resultados: As qualidades analisadas, principalmente velocidade de movimento, velocidade de balanço, agilidade e resistência para balanço rápido e movimento rápido mostraram um diferencial significativo após a intervenção. A velocidade de resposta concentrou-se no centro de equilíbrio corporal. A resistência para a velocidade foi incrementada pelos membros inferiores. Após o treinamento da velocidade de reação visual, a capacidade do grupo experimental de enfrentar os ataques sobre a rede foi estatisticamente superior (P<0,05). Conclusão: O treinamento da velocidade de reação dos jogadores de vôlei pode ser aprimorado com o protocolo proposto. Os jogadores de vôlei devem reforçar o treinamento da velocidade de reação em seu treinamento diário afim de uma melhor capacidade de atuação próxima à rede. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El voleibol es un deporte de equipo y sus características ganadoras están determinadas en gran medida por el enfrentamiento en la red. Los jugadores de voleibol deben esforzarse constantemente por mejorar la cooperación en equipo, la capacidad defensiva y las habilidades de velocidad para mejorar su capacidad de actuación sobre la red. Objetivo: Establecer un modelo de carga de intensidad fija para el entrenamiento de velocidad sobre la respuesta visual del deportista en el voleibol. Métodos: Este trabajo selecciona a varios jugadores de voleibol como sujetos de investigación, divididos aleatoriamente en dos grupos (grupos de entrenamiento regular y grupos experimentales). El grupo experimental tuvo el diferencial de ser sometido a un protocolo de entrenamiento de la velocidad de respuesta visual basado en el entrenamiento rutinario. Se emplearon estadísticas matemáticas para analizar la velocidad de reacción y la capacidad defensiva de los atletas contra la red. Resultados: Las cualidades analizadas, principalmente la velocidad de movimiento, la velocidad de balanceo, la agilidad y la resistencia para el balanceo rápido y el movimiento rápido mostraron un diferencial significativo después de la intervención. La velocidad de respuesta se concentró en el centro de equilibrio del cuerpo. La resistencia para la velocidad se incrementó en los miembros inferiores. Tras el entrenamiento de la velocidad de reacción visual, la capacidad del grupo experimental para afrontar los ataques sobre la red fue estadísticamente superior (P<0,05). Conclusión: El entrenamiento de la velocidad de reacción de los jugadores de voleibol puede mejorar con el protocolo propuesto. Los jugadores de voleibol deben reforzar el entrenamiento de la velocidad de reacción en su formación diaria para mejorar su capacidad de actuación cerca de la red. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

The effectiveness of ultrasound-guided core needle biopsy in detecting lymph node metastases in the axilla in patients with breast cancer: systematic review and meta-analysis

Abstract Objective: This study aimed to perform a meta-analysis to investigate the diagnostic safety and accuracy of Ultrasound-Guided Core Needle Biopsy (US-CNB) Axillary Lymph Nodes (ALNs) region in patients with Breast Cancer (BC). Methods: The authors searched the electronic databases PubMed, Scopus, Embase, and Web of Science for clinical trials about US-CNB for the detection of ALNs in breast cancer patients. The authors extracted and pooled raw data from the included studies and performed statistical analyses using Meta-DiSc 1.4 and Review Manager 5.3 software. A random effects model was used to calculate the data. At the same time, data from the Ultrasound-guided Fine-Needle Aspiration (US-FNA) were introduced for comparison with the US-CNB. In addition, the subgroup was performed to explore the causes of heterogeneity. (PROSPERO ID: CRD42022369491). Results: In total, 18 articles with 2521 patients were assessed as meeting the study criteria. The overall sensitivity was 0.90 (95% CI [Confidence Interval], 0.87‒0.91; p = 0.00), the overall specificity was 0.99 (95% CI 0.98‒ 1.00; p = 0.62), the overall area under the curve (AUC) was 0.98. Next, in the comparison of US-CNB and US-FNA, US-CNB is better than US-FNA in the diagnosis of ALNs metastases. The sensitivity was 0.88 (95% CI 0.84‒ 0.91; p = 0.12) vs. 0.73 (95% CI 0.69‒0.76; p = 0.91), the specificity was 1.00 (95% CI 0.99‒1.00; p = 1.00) vs. 0.99 (95% CI 0.67‒0.74; p = 0.92), and the AUC was 0.99 vs. 0.98. Subgroup analysis showed that heterogeneity may be related to preoperative Neoadjuvant Chemotherapy (NAC) treatment, region, size of tumor diameter, and the number of punctures. Conclusion: US-CNB has a satisfactory diagnostic performance with good specificity and sensitivity in the preoperative diagnosis of ALNs in BC patients.

Effect of physical exercise on medical rehabilitation treatment of depression / Efeito de exercícios físicos no tratamento médico para reabilitação da depressão / Efectos de ejercicios físicos en el tratamiento médico para rehabilitación de la depresión

ABSTRACT Introduction: Depression is a common disease worldwide. The main treatment methods currently include medication, psychotherapy, and physical therapy. However, due to limitations in treatment methods, treatment compliance is poor. Participating in various sports is very effective in treating depression. Objective: To verify the influence of sports on the condition of depression and on the effect of its clinical treatment. Methods: The article selected a total of 60 hospitalized patients with depression who were randomly divided into two groups: an experimental group and a control group. The control group took antidepressant drugs for eight weeks, and the experimental group took the drugs with the supplementary practice of sports. The two groups of patients were tested for serum β-endorphin (β-EP) levels before and after treatment, and HAMD scores were performed. Results: The scores and serum β-endorphin (β-EP) levels of the two groups of patients were different, showing that effect of the treatment was better in the experimental group. Conclusion: Physical exercise therapy can mobilize the enthusiasm of patients with depression. This treatment plan increases treatment efficiency and is suitable for long-term clinical promotion and application. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução: A depressão é uma doença comum em todo o mundo. Os principais métodos de tratamento atuais incluem medicação, psicoterapia e fisioterapia. Contudo, graças às limitações dos métodos de tratamento, a aderência a eles é pequena. Participar de vários esportes é uma maneira eficiente de se tratar a depressão. Objetivo: Verificar a influência de esportes na condição depressiva e seu efeito no tratamento clínico da doença. Método: Selecionou-se 60 pacientes hospitalizados, que foram aleatoriamente divididos em dois grupos: um grupo experimental e um grupo controle. O grupo controle tomou antidepressivos por oito semanas, e o grupo experimental usou os medicamentos somados à prática de esportes. Testou-se os níveis de beta-endorfina (β-EP) dos dois grupos antes e depois do tratamento, e a pontuação na escala HAMD foi avaliada. Resultados: Houve diferença na pontuação e nos níveis de β-EP entre os grupos, indicando que o efeito do tratamento foi melhor no grupo experimental. Conclusão: A terapia com exercício físico é capaz de mobilizar o entusiasmo dos pacientes com depressão. Esse plano de tratamento aumenta a eficiência da terapia e é adequado para promoção e aplicação a longo prazo. Nível de evidência II; Estudos terapêuticos - investigação de resultados de tratamento.

RESUMEN Introducción: La depresión es una enfermedad común en todo el mundo. Los principales métodos de tratamiento actuales incluyen medicación, psicoterapia y fisioterapia. Sin embargo, gracias a las limitaciones de los métodos de tratamiento, la adherencia a los mismos es baja. Practicar varios deportes es una forma eficaz de tratar la depresión. Objetivo: Verificar la influencia de los deportes en la condición depresiva y su efecto en el tratamiento clínico de la enfermedad. Método: Se seleccionaron 60 pacientes hospitalizados, que fueron aleatoriamente divididos en dos grupos: un grupo experimental y un grupo control. El grupo control tomó antidepresivos por ocho semanas, y el grupo experimental usó los medicamentos sumados a la práctica de deportes. Se analizaron los niveles de beta-endorfina (β-EP) de ambos grupos antes y después del tratamiento, y se evaluó la puntuación de la escala HAMD. Resultados: Hubo diferencia en la puntuación y en los niveles de β-EP entre los grupos, indicando que el efecto del tratamiento fue mejor en el grupo experimental. Conclusión: La terapia con ejercicio físico es capaz de movilizar el entusiasmo de los pacientes con depresión. Este plan de tratamiento aumenta la eficacia de la terapia y es adecuado para la promoción y aplicación a largo plazo. Nivel de evidencia II; Estudios terapéuticos - investigación de resultados de tratamiento.

Application of Mivacurium in Fast-Track Anesthesia for Transthoracic Device Closure of Ventricular Septal Defects in Children

Abstract Introduction: The objective of this study was to investigate the effect of mivacurium in the application of fast-track anesthesia for transthoracic device closure of ventricular septal defects (VSDs) in children. Methods: The data of 108 children who underwent transthoracic device closure of VSDs from December 2018 to June 2020 were recorded and analyzed. All children were divided into group M (mivacurium group, n=55) and group C (cisatracurium group, n=53) according to the different muscle relaxant drug used. Results: No statistically significant differences in general preoperative data, intraoperative hemodynamic changes, or the incidence of adverse reactions were noted between the two groups (P>0.05). However, the intubation condition rating of children in group M was better than that in group C. The onset time, duration of clinical action and recovery index of the muscle relaxant, postoperative mechanical ventilation duration, and length of intensive care unit stay in group M were significantly lower than those in group C (P<0.05). Conclusion: It is safe and feasible to use mivacurium as a muscle relaxant in children undergoing fast-track cardiac anesthesia during transthoracic device closure of VSDs.

Structure and character analysis of cotton response regulator genes family reveals that GhRR7 responses to draught stress

BACKGROUND: Cytokinin signal transduction is mediated by a two-component system (TCS). Two-component systems are utilized in plant responses to hormones as well as to biotic and abiotic environmental stimuli. In plants, response regulatory genes (RRs) are one of the main members of the two-component system (TCS). METHOD: From the aspects of gene structure, evolution mode, expression type, regulatory network and gene function, the evolution process and role of RR genes in the evolution of the cotton genome were analyzed. RESULT: A total of 284 RR genes in four cotton species were identified. Including 1049 orthologous/paralogous gene pairs were identified, most of which were whole genome duplication (WGD). The RR genes promoter elements contain phytohormone responses and abiotic or biotic stress-related cis-elements. Expression analysis showed that RR genes family may be negatively regulate and involved in salt stress and drought stress in plants. Protein regulatory network analysis showed that RR family proteins are involved in regulating the DNA-binding transcription factor activity (COG5641) pathway and HP kinase pathways. VIGS analysis showed that the GhRR7 gene may be in the same regulatory pathway as GhAHP5 and GhPHYB, ultimately negatively regulating cotton drought stress by regulating POD, SOD, CAT, H2O2 and other reactive oxygen removal systems. CONCLUSION: This study is the first to gain insight into RR gene members in cotton. Our research lays the foundation for discovering the genes related to drought and salt tolerance and creating new cotton germplasm materials for drought and salt tolerance.

Molecular structures and functional exploration of NDA family genes respond tolerant to alkaline stress in Gossypium hirsutum L

BACKGROUND: The internal NAD(P)H dehydrogenase (NDA) gene family was a member of the NAD(P)H dehydrogenase (ND) gene family, mainly involved in the non-phosphorylated respiratory pathways in mitochondria and played crucial roles in response to abiotic stress. METHODS: The whole genome identification, structure analysis and expression pattern of NDA gene family were conducted to analyze the NDA gene family. RESULTS: There were 51, 52, 26, and 24 NDA genes identified in G. hirsutum, G. barbadense, G. arboreum and G. raimondii, respectively. According to the structural characteristics of genes and traits of phylogenetic tree, we divided the NDA gene family into 8 clades. Gene structure analysis showed that the NDA gene family was relatively conservative. The four Gossypium species had good collinearity, and segmental duplication played an important role in the evolution of the NDA gene family. Analysis of cis-elements showed that most GhNDA genes contained cis-elements related to light response and plant hormones (ABA, MeJA and GA). The analysis of the expression patterns of GhNDA genes under different alkaline stress showed that GhNDA genes were actively involved in the response to alkaline stress, possibly through different molecular mechanisms. By analyzing the existing RNA-Seq data after alkaline stress, it was found that an NDA family gene GhNDA32 was expressed, and then theGhNDA32 was silenced by virus-induced gene silencing (VIGS). By observing the phenotype, we found that the wilting degree of silenced plants was much higher than that of the control plant after alkaline treatment, suggesting that GhNDA32 gene was involved in the response to alkaline stress. CONCLUSIONS: In this study, GhNDAs participated in response to alkaline stress, especially NaHCO3 stress. It was of great significance for the future research on the molecular mechanism of NDA gene family in responding to abiotic stresses.

Social anxiety and associated factors among graduate students under the normalization of epidemic prevention and control / 中国学校卫生

Objective@#To understand social anxiety and relevant factors among graduate students under the normalization stage of COVID-19 epidemic prevention and control.@*Methods@#Using convenience sampling method, an online questionnaire survey on graduate students from 5 universities in Jiangsu Province was conducted. Measurements used in the survey includes General Self Efficacy Scale (GSES), General Alienation Scale (GAS), Interaction Anxiousness Scale (IAS) and self made survey for basic information and household living conditions.@*Results@#The overall score of graduate students self efficacy was (2.58±0.50). Average score was (30.68±6.22) for alienation, and (47.55±8.77) for interaction anxiety, with detection rate of social anxiety being 43.96%. Increased dependence on smartphones and electronic devices ( OR=1.86, 95%CI =1.32-2.61) and high alienation score (medium level: OR=2.06, 95%CI =1.45-2.92; high level: OR=5.19, 95%CI =1.00-27.00) were positively correlated with social anxiety. Increased communication with friends ( OR=0.65, 95%CI =0.47-0.90 and high self efficacy (medium level: OR= 0.37 , 95%CI =0.21-0.66; high level: OR=0.15, 95%CI =0.08-0.30) were negatively correlated with social anxiety.@*Conclusion@#At the normalization stage of COVID-19 epidemic prevention and control, social anxiety of graduate students is one of the mental health issues which need further attention. Participation in peer support helps prevent social anxiety through developing self efficacy, alleviating individual alienation, and reducing dependence on electronic devices among graduate students.

Effects of cumulative ecological risk on healthy lifestyle among college students / 中国学校卫生

Objective@#To investigate the effects and patterns of cumulative ecological risk on currrent and 6 month follow up healthy lifestyle among college students.@*Methods@#A total of 1 156 freshmen and sophomores students randomly selected from 3 universities in Hebei Province were administered with College Students Health Lifestyle Scale and the Cumulative Ecological Risk Scale in November 2020 and May 2021 respectively. SPSS 24.0 statistical software was used for hierarchical regression analysis.@*Results@#Cumulative ecological risk was both negatively associated with current healthy lifestyle ( β =-0.29, P <0.01), and healthy lifestyle 6 month after ( β =-0.11, P <0.01). The effects of cumulative ecological risks on current healthy lifestyle showed non linear pattern, with healthy lifestyle rapidly decline after accumulated more than 4 factors. The effects of umulative ecological risks on 6 month follow up healthy lifestyle showed a linear pattern. No moderating effect of gender was observed in the linear relationship between the cumulative ecological risk and 6 month follow up healthy lifestyle( β =-0.02， P >0.05), however, significant moderating effects of gender was found in the nonlinear relationship between cumulative ecological risk and current healthy lifestyle( β =0.12， P < 0.01).@*Conclusion@#Cumulative ecological risk is negatively associated with healthy lifestyle of college students cross sectionally and longitudinally, in a non linear and linear association pattern, respectively. Gender plays a moderating effect on the cross sectional asscociation between cumulative ecological risk and healthy lifestyle.

Investigation on outbreaks of acute respiratory tract infection caused by respiratory syncytial virus in kindergartens in Hangzhou / 中国学校卫生

Objective@#In order to analyze the characteristics of the outbreak of acute respiratory tract infection in children caused by respiratory syncytial virus(RSV).@*Methods@#The field epidemiological investigations were conducted for the two outbreaks in kindergartens in Hangzhou. Data were analyzed by descriptive method. Samples with positive respiratory syncytial virus nucleic acid were sequenced using PCR.@*Results@#The two outbreaks occurred in kindergartens. There were 21 cases in kindergarten A, lasting 11 days, and 43 cases in kindergarten B, lasting 33 days. The epidemic curve showed a proliferation pattern. The cases were concentrated in nurseries and K1 classes, primarily among children aged 2-4 years. The most common symptoms were fever and cough, mainly upper respiratory tract infection, and no severe cases were found. Upper respiratory tract samples were collected and detected as positive for RSV. Four samples were sequenced and identified as subgroup B.@*Conclusion@#During the outbreak of acute respiratory infection in kindergartens, respiratory syncytial virus should be given primary consideration in the process of identification of the outbreak caused by other respiratory infections, and strictly control measures should be taken to reduce the long term impact of the epidemic.

Aplication of digitally modified fence technique in vertical bone reconstruction / 口腔疾病防治

Objective@#To propose a digitally modified and guided bone regeneration technique supported by a nonabsorbable titanium plate and explore its effect on vertical bone regeneration.@*Methods@#A total of 8 patients with severe vertical bone defects in the edentulous area who wanted to be treated with implants were included in this study. A digitally modified and guided bone regeneration technique supported by a nonabsorbable titanium plate (fence technique) was used for bone augmentation. The patient's jaw, dentition, and soft tissue data were obtained for prosthetically guided implantation and bone regeneration. After virtual bone augmentation, a model of the jaw was obtained through 3D printing technology, and the titanium plate was bent accordingly. The virtual design was transformed through the template (including the base template and the attachment of a periosteal screw and bone block), so the actual osteogenesis space consistent with the design could be realized in the operation. Guided bone regeneration was performed according to the improved procedure and technical process. After 6 ~ 8 months of bone augmentation, cone beam CT was taken to evaluate the effect of bone augmentation. The implant was implanted according to the initial implant design, and bone tissue was obtained for HE and Masson staining. @* Results@#After 6 ~ 8 months of bone augmentation, the vertical linear bone increment reached (5.44 ± 1.73) mm. The implant was implanted according to the initial implant design, and the bone tissue was obtained for histological examination to show the formation of new bone.@*Conclusion@#Digital improved fence technique can simplify the preoperative and surgical procedures, and obtain good vertical bone augmentation results. In short, it is a kind of vertical bone augmentation technique worth popularizing and applying.

PARP1 promotes proliferation and 5-FU resistance of gastric cancer AGS cells by regulating N-glycosyltransferase FUT8 / 中国肿瘤生物治疗杂志

@#[Abstract] Objective: To investigate the effect of polyadenosine diphosphate ribose polymerase 1 (PARP1) on the proliferation and 5-FU resistance of gastric cancer cells and its potential mechanism. Methods: The tumor tissues and corresponding paracancerous tissues of 72 patients with gastric cancer who were treated in Central Hospital of Enshi Tujia and Miao Autonomous Prefecture from May 2018 to December 2019 were collected. AGS cells were transfected with siFUT8 to knock down FUT8 gene expression. qPCR and immunohistochemistry were used to detect the expression of PARP1 in gastric cancer and adjacent tissues. CCK-8, flow cytometry, and colony formation assay were employed to detect the effects of AG14361 on the proliferation, apoptosis and colony formation of AGS cells. MTT assay was used to detect the effect of AG14361 on the 5-FU sensitivity of gastric cancer cells. The overall distribution of differential genes in AGS cells treated with AG14361 was analyzed by mRNA sequencing, and related signaling pathways were analyzed by KEGG enrichment. qPCR and WB were used to detect the expression of α-1,6-fucosyltransferase (FUT8) in AGS cells and the interference effect of FUT8. CCK-8, flow cytometry, and colony formation assay were employed to detect the effects of AG14361 on the proliferation, apoptosis, and colony formation of AGS cells disturbed by siFUT8. Results: Compared with paracancer tissues, PARP1 expression was significantly increased in gastric cancer tissues (P<0.001). AG14361 can significantly inhibit the proliferation and colony formation of AGS cells, thus promoting the apoptosis of AGS cells (all P<0.01). AG14361 treatment reduced the IC50 of 5-FU against gastric cancer cells, especially against AGS cells, with IC50 decreased by more than 60%. mRNA sequencing results showed that FUT8 was a key glycosyltransferase of AG14361 in inhibiting the proliferation of AGS cells (P<0.05). Compared with the siNC group, treatment of AG14361 with IC50 significantly reversed the promotion of AGS cells proliferation caused by inerference with FUT8, promoted apoptosis and BAX protein expression, decreased Bcl2 protein expression and inhibited the increase in AGS cell colony formation caused by interference with FUT8 (all P<0.01). Conclusion: PARP1 can promote malignant transformation of gastric cancer cells by regulating N-glycosyltransferase FUT8. AG14361 can enhance the chemotherapy sensitivity of 5-FU, and PARP1 may become a potential target for gastric cancer treatment.

A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching / 药物分析学报

Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and rice.Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs.DNA oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal amplification.The detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the fluorophore.The resulting fluorescence signal en-ables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concen-tration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,respectively.The mean recovery ranged between 80.3％and 110.8％with relative standard deviations of 7.3％-17.6％,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

Relationship between prepregnancy body mass index weight gain during pregnancy and offspring body composition during preschool age / 中国学校卫生

Objective@#To explore the relationship between prepregnancy body mass index, weight gain during pregnancy with preschool obesity and body composition in offspring, so as to provide evidence for gestational weight gain and childhood obesity prevention.@*Methods@#A total of 1 333 preschool children were recruited from 3 kindergartens in Tianjin from September to December 2020. Structured questionnaire was used to collect children s lifestyle information. Height, weight and body fat mass of children were assessed, and body fat percentage (FM%), fat mass index (FMI) and non fat mass index (FFMI) were calculated. Maternal medical records were collected and the mothers were grouped according to their prepregnancy weight status and weight gain during pregnancy. χ 2 test, t test, linear regression model and Logistic regression were used to analyze the differences of obesity and body composition among different groups.@*Results@#The prevalence of overweight and obesity in preschoolers was 12.7% and 7.7%. After adjusting maternal age and delivery, gestational age, gender, age and lifestyle of children, the correlation between maternal pre pregnancy BMI,gestational weight gain with obesity and body composition indexes of children in preschool age was statistically significant ( P <0.05). For mothers with normal weight before pregnancy, excessive weight gain during pregnancy increased risk of high FM% and high FMI in offspring ( OR=1.81, 1.68, P <0.05). There was no significant correlation between maternal weight gain during pregnancy with offspring obesity and body composition among mothers with prepregnant overweight or obesity.@*Conclusion@#Maternal weight status before pregnancy and weight gain during pregnancy are correlated with obesity and body composition in the preschool age of offspring. It is suggested that mothers should maintain appropriate weight status before and during pregnancy.

The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis

Abstract Background: Postoperative cognitive dysfunction is widely recognized as severe postoperative central nervous dysfunction and has a significant impact on the 'patient's physical and mental health. Methods: Postoperative models of tibial fracture in aged rats were established, including the control group, model group, CCL11 protein injection group, and saline injection group. Morris water maze test was used to detect the behavioral characteristics of rats. Enzyme-Linked Immunosorbent Assay was used or determine the content of CCL11 and CXCL10. Immunofluorescence staining was used to detect the distribution of CD14+CD163+macro-phages in colon tissues and CD11b+CCR3+microglia cells in hippocampal tissues. Western blot analyzed NOX1 and STAT3 expression in hippocampus tissues. Results: Water maze test results confirmed severe cognitive impairment in CCL11 rats. The content of CCL11 and CXCL10 in the CCL11 group was much higher than that of the model group. The distribution of macrophage and microglia cells in the CCL11 model group was greater than that in the model group and the saline group. The expression of NOX1 and STAT3 in the CCL11 group was higher compared with the model group. Conclusion: Abnormal macrophage function and excessive CCL11 secretion were observed in the rats with lower limb fractures after surgery. Postoperative central inflammation in rats with lower limb fracture induced postoperative cognitive dysfunction through the gut-brain axis molecular mechanism.

Extracorporeal Membrane Oxygenation-Related Nosocomial Infection after Cardiac Surgery in Adult Patients

Abstract Introduction: The evaluation of extracorporeal membrane oxygenation-related nosocomial infection (ECMO-related NI) in a homogeneous cohort remains scarce. This study analyzed ECMO-related NI in adult patients who have undergone cardiac surgery. Methods: From January 2012 to December 2017, 322 adult patients who have received ECMO support after cardiac surgery were divided into the infection group (n=131) and the non-infection group (n=191). ECMO-related NI was evaluated according to demographic data, surgical procedures, and ECMO parameters. Results: The incidence of ECMO-related NI was 85.4 cases per 1000 ECMO days. Acinetobacter baumannii was the most common pathogen causing blood stream infection and respiratory tract infection. Prolonged duration of surgery (P=0.042) and cardiopulmonary bypass assist (P=0.044) increased the risk of ECMO-related NI. Body mass index (odds ratio [OR]: 1.077; 95% confidence interval [CI]: 1.004-1.156; P=0.039) and duration of ECMO support (OR: 1.006; 95% CI: 1.003-1.009; P=0.0001) were the independent risk factors for ECMO-related NI. Duration of ECMO support > 144 hours (OR: 2.460; 95% CI: 1.155-7.238; P<0.0001) and ECMO-related NI (OR: 3.726; 95% CI: 1.274-10.895; P=0.016) increased significantly the risk of in-hospital death. Conclusion: Prolonged duration of ECMO support was an independent risk factor for NI. Surgical correcting latent causes of cardiopulmonary failure and shortening duration of ECMO whenever possible would reduce susceptibility to NI.